preparation of hydrogels using human amniotic membrane and their characterization.

by:Max Apparel     2020-06-20
Abstract: Human amniotic fluid, as a temporary biological wound dressing, is still a useful and cost-effective means to treat burns in developing countries.
In the current research work, efforts have been made to cross-link the protein with Amber and then with Poly oh acetate (PHEMA)
It was used as the initiator of potassium 2 and Sodium 2.
The developed products were characterized by water absorption capacity, mechanical strength, infrared spectrum and thermal weight analysis.
The results show that the water absorption capacity and tensile strength of natural amniotic fluid are higher than that of all other samples.
The infrared spectrum has clearly shown the cross-union and grafting of amniotic fluid with propolis and PHEMA respectively.
It was observed from the thermal weight analysis that the cross-linked amniotic fluid had more thermal stability than the natural membrane.
Further, the grafting rate of the Contact film on PHEMA was higher.
Although the mechanical strength and water absorption capacity of the chemically modified human amniotic fluid are lower than that of the natural amniotic fluid, it can also be used as a gel.
Since the material is cross-connected with propolis, it can be stored for a long time.
Human amniotic fluid is still beneficial as a temporary biological wound dressing and is costly
Effective means of treating burns in developing countries. Maral et al [1]
The efficacy of human amniotic fluid, which has been preserved in glycerin for a long time, as a temporary biological dressing was studied.
It is found that the preserved glycerin amniotic fluid is as effective as the fresh amniotic fluid and can be kept for a longer time. Chang et al [2]
Describes a kind of in-60[degrees]
More than six months. Thomson et al [3]
Amniotic fluid was used and it is recommended that amniotic fluid is a cost effective biological dressing that can be used as an instant dressing in shallow second degree burns.
Several investigators tried to use fresh human amniotic fluid as a burn dressing [4-8].
Rao et al described a new method for drying and sterilization of amniotic fluid that can be preserved for more than 9 months9].
Sastry et al [10]
Amniotic fluid and Poly β-acetate (PHEMA)
Through the oxidation-reduction initiation system containing hydrogen peroxide and ammonium sulfate.
In this study, we worked hard to prepare and characterize the gel of human amniotic fluid using PHEMA, using different initiator, using potassium and sodium pyroacetate.
These gels are expected to absorb the wound fluid and store better.
Human amniotic fluid was initially cross-linked with gelatin that was subsequently aggregated with PHEMA.
Materials and methods collect human placenta from a nearby maternity hospital, manually remove amniotic fluid, thoroughly clean the membrane with cold tap water until there is no blood.
Then treat the film with 0.
05 M sodium acetate solution for two hours, remove the residual blood in the sheep water.
Cross-linking of amniotic fluid: As mentioned earlier, amniotic fluid is cross-linking with Amber10].
Grafting polymerization of PHEMA on sheep water: grafting polymerization was carried out in a 250 ml round bottom flask.
Soak amniotic fluid in 100 ml water for about 1 hour and add 25 ml Initiator solution [
Potassium content (1. 8x[10. sup. -3]M)
Sodium Jiaozuo (1. 2x[10. sup. -3]M)]
Followed by 5 ml PHEMA.
The experiment was conducted at room temperature for 2 hours.
Crude products were repeatedly extracted with ethanol, PHEMA homopolymers were removed after 72 hours, and then dried at room temperature.
Grafting and co-polymerization experiments were carried out with this amniotic fluid and cross-linked amniotic fluid.
Products named as follows. AM--
Such amniotic fluid-G--
Gelatin AM-cross-linked amniotic fluidPHEMA--
Uncross-linked amniotic fluid graft aggregated with phema am-G-PHEMA--
Cross-linked amniotic fluid graft characterized by PHEMA: Product Analysis (AM, AM-G, AM-PHEMA, AM-G-PHEMA)
The study of water absorption capacity, tensile strength, infrared spectrum and thermal weight analysis was carried out.
Water absorption capacity: the water absorption capacity of AM and AM-G, AM-PHEMA, AM-
GPHEMA is determined according to the method [followed] by Rao et al. 11].
A small piece of each sample of known weight, dried at a constant weight of 100 [degrees]
C is allowed to expand in distilled water at room temperature (22[degrees]C).
Dry the sample with the filter paper and then weigh the sample accurately to determine the swelling weight of the sample.
The weight of the expansion piece is recorded every 1, 2, 3 and 24 hours, and the percentage of sample expansion for a given time is calculated based on the following equation E 7 (5), 354-356. [4. ]
Dr. Walker AB, Kuni, Alan JE.
Use fresh amniotic fluid as a burn dressing.
Pediatrician, 12 years old (3), 391-395 (1977). [5. ]
Metarn, Bali, Bhatnagar, Rai page.
Human amniotic fluid is used as a biological dressing for burn wounds.
Indian Medical Association. , 81 (11-12), 189-191 (1983). [6. ]
Maganatra, Durani KM.
Methods for obtaining and preparing fresh human amniotic fluid for clinical use.
Pakistan Medical Association. , 46 (6), 126-128 (1996). [7. ]Ramakrishnan K. M, Jayaram V.
Treatment of partial thickness burns of amniotic fluid: an economical and effective treatment in developing countries. Burns, 23(1), S33-36 (1997). [8. ]
Shanna SC, Bagree MM, Bhat AL, Banga BB, Singh MP.
Amniotic fluid is an effective burn dressing. Jpn J Surg,15(2), 140-143 (1985). [9. ]
Chandtv, Chandra Kalan V
Dry people, cattle and sheep water are used as biological dressings. Arch Surg, 116(7), 891-896 (1981). [10. ]
Panuranga Rao K.
Gel aggregation based on amniotic fluid collagen (
Ester Base)
J. Bioactive and compatible polymer, 430-438 (1990). [11. ]
KP Rao Joseph Clarke, Nayudamma Y.
CericIon-grafting vinyl monomer onto modified collagen
Study on grafting sites. Leath. Sci. , 16, 401-408 (1969).
Saraswathy, S. E. Noorjahan, S. Chinni Krishnan, Ganga Radhakrishnan, T. P. Sastry Central Institute of leather-
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